recombinant human cox2  (R&D Systems)

Journal: Molecules

Article Title: An Investigation into the Interaction between Double Hydroxide-Based Antioxidant Benzophenone Derivatives and Cyclooxygenase 2

doi: 10.3390/molecules26216622

Figure Lengend Snippet: Sensograms for the interaction of 12 compounds with cyclooxygenases 2 (COX2) obtained with biolayer interferometry (BLI); ( a ) All 12 compounds were initially assayed at a single concentration of 100 μM. ( b – d ) The results showed a medium-high affinity of compounds DB2 ( b ), SC2 ( c ) and YB2 ( d ) to COX2.

Article Snippet: Recombinant active human COX2 was purchased from Sino Biological Co. (Beijing, China).

Techniques: Concentration Assay

Journal: Molecules

Article Title: An Investigation into the Interaction between Double Hydroxide-Based Antioxidant Benzophenone Derivatives and Cyclooxygenase 2

doi: 10.3390/molecules26216622

Figure Lengend Snippet: 3D putative binding complexes and hydrogen bonding of compounds DB2 ( a , b ), SC2 ( c , d ) and YB2 ( e , f ) with the COX2 enzyme (PDB code 5f1a). (COX2 is shown as cyan ribbon; The ligand surface is indicated in purple; Compounds DB2, SC2 and YB2 are shown in capped sticks models; The amino acids involved in hydrogen bond contact are shown in line models; Hydrogen bonding are yellow lines).

Article Snippet: Recombinant active human COX2 was purchased from Sino Biological Co. (Beijing, China).

Techniques: Binding Assay

Journal: Molecules

Article Title: An Investigation into the Interaction between Double Hydroxide-Based Antioxidant Benzophenone Derivatives and Cyclooxygenase 2

doi: 10.3390/molecules26216622

Figure Lengend Snippet: Spectra of COX2 (6.0 × 10 −8 mol∙L −1 ) fluorescence quenching upon binding to compounds DB2 ( a , b ), SC2 ( c , d ) or YB2 ( e , f ) (1→7: 0, 0.75, 1.50, 2.25, 3.00, 3.75, 4.50 × 10 −5 mol∙L −1 ) at 298 K and 310 K.

Article Snippet: Recombinant active human COX2 was purchased from Sino Biological Co. (Beijing, China).

Techniques: Fluorescence, Binding Assay

Journal: Molecules

Article Title: An Investigation into the Interaction between Double Hydroxide-Based Antioxidant Benzophenone Derivatives and Cyclooxygenase 2

doi: 10.3390/molecules26216622

Figure Lengend Snippet: Stern-Volmer plots for COX2-DB2 ( a ), COX2-SC2 ( b ) and COX2-YB2 ( c ) at 298 K and 310 K; Double logarithmic plots for COX2-DB2 ( d ), COX2-SC2 ( e ) and COX2-YB2 ( f ) at 298 K and 310 K; The overlap between COX2 (5.0 × 10 −8 mol∙L −1 ) fluorescence spectra (solid line) and compounds DB2 ( g ), SC2 ( h ) or YB2 ( i ) (5.0 × 10 −8 mol∙L −1 ) absorbance spectra (short dot).

Article Snippet: Recombinant active human COX2 was purchased from Sino Biological Co. (Beijing, China).

Techniques: Fluorescence

Journal: Molecules

Article Title: An Investigation into the Interaction between Double Hydroxide-Based Antioxidant Benzophenone Derivatives and Cyclooxygenase 2

doi: 10.3390/molecules26216622

Figure Lengend Snippet: The Stern-Volmer quenching constant ( Ksv ), bimolecular quenching constant ( Kq ) and binding constant ( K A ) for the interaction of compounds DB2, SC2 and YB2 with COX2 at 298 K and 310 K.

Article Snippet: Recombinant active human COX2 was purchased from Sino Biological Co. (Beijing, China).

Techniques: Binding Assay, Control

Journal: Molecules

Article Title: An Investigation into the Interaction between Double Hydroxide-Based Antioxidant Benzophenone Derivatives and Cyclooxygenase 2

doi: 10.3390/molecules26216622

Figure Lengend Snippet: Thermodynamic parameters, the effective spectral overlap ( J ) and the separation distance ( r ) for the interaction of compounds DB2, SC2 and YB2 with COX2 at 298 K and 310 K.

Article Snippet: Recombinant active human COX2 was purchased from Sino Biological Co. (Beijing, China).

Techniques:

Journal: Molecules

Article Title: An Investigation into the Interaction between Double Hydroxide-Based Antioxidant Benzophenone Derivatives and Cyclooxygenase 2

doi: 10.3390/molecules26216622

Figure Lengend Snippet: Synchronous fluorescence spectra of COX2 (6.0 × 10 −8 mol∙L −1 ) in the presence of different concentrations of compounds DB2 ( a , b ), SC2 ( c , d ) and YB2 ( e , f ) (1→7: 0, 0.75, 1.50, 2.25, 3.00, 3.75, 4.50 × 10 −5 mol∙L −1 ) at 298 K.

Article Snippet: Recombinant active human COX2 was purchased from Sino Biological Co. (Beijing, China).

Techniques: Fluorescence

Journal: Molecules

Article Title: An Investigation into the Interaction between Double Hydroxide-Based Antioxidant Benzophenone Derivatives and Cyclooxygenase 2

doi: 10.3390/molecules26216622

Figure Lengend Snippet: Three-dimensional fluorescence contour spectra of COX2 (5.0 × 10 −8 mol∙L −1 ) in the absence ( a ) and presence of compounds DB2 ( b ), SC2 ( c ) or YB2 ( d ) (4.50 × 10 −5 mol∙L −1 ) at 298 K.

Article Snippet: Recombinant active human COX2 was purchased from Sino Biological Co. (Beijing, China).

Techniques: Fluorescence

Journal: Molecules

Article Title: An Investigation into the Interaction between Double Hydroxide-Based Antioxidant Benzophenone Derivatives and Cyclooxygenase 2

doi: 10.3390/molecules26216622

Figure Lengend Snippet: The characteristic parameters of three-dimensional fluorescence spectra.

Article Snippet: Recombinant active human COX2 was purchased from Sino Biological Co. (Beijing, China).

Techniques: Fluorescence